Germination Timing, Plant Chemicals Screening and Assessment of Antioxidant Activity on Various Microgreens Vegetables Employing the DPPH (2,2-diphenyl-1- picrylhydrazyl) Technique

Authors

  • Hilmi Oktari Universitas Andalas
  • Warnita Warnita Universitas Andalas
  • Ryan Budi Setiawan Universitas Andalas

DOI:

https://doi.org/10.25077/jijcs.v8i3.247

Keywords:

agriculture, antioxidant, phytochemical, microgreen, vegetables

Abstract

The decline of agricultural land and the growth of urban areas have given rise to
the idea of gardening in confined spaces. Urban agriculture is becoming more
popular alongside the increasing recognition of healthy living. Microgreens are a
type of vegetable that looks like sprouts but has a longer growth cycle, larger
leaves, and a deeper green hue. These vegetables can be picked 7 to 21 days after
planting. Nearly all varieties of microgreens contain much higher concentrations
of bioactive compounds and antioxidants compared to fully grown leaves. This
study aimed to assess the timing of germination, phytochemical composition, and
antioxidant properties of microgreens. This research employed a fully
randomized design (FRD) featuring a single treatment factor: microgreens, which
encompassed green lettuce, red lettuce, green spinach, red spinach, and mustard
greens. Data from observations were examined using a 5% variance test. The
findings showed that gradual alterations in seed morphology mark germination
phenology. Phytochemical analysis revealed the presence of tannins, saponins,
flavonoids, phenolic compounds, and steroids. The greatest antioxidant activity
was observed in mustard greens, boasting an IC50 value of 226.01 ppm. Thus, it
can be determined that mustard greens possess the greatest antioxidant activity
among microgreens.

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References

[1] Azalia, D., Rachmawati, I., Zahira, S., Andriyani, F., Sanini, T.

M., Supriyatin & Aulya N, R. (2023). Uji kualitatif senyawa aktif

flavonoid dan terpenoid pada beberapa jenis tumbuhan

Fabaceae dan Apocynaceae di kawasan TNGPP bodogol.

Jurnal Biologi Makassar,8(1),32-43.https://journal.unhas.ac.id/index.php/bioma

[2] Cahyani, N. W., Izzah, N. L., Irawanto, R. (2023). Fenologi dan

karakterisasi biji tumbuhan air Nelumbo nucifera, Thalia

geniculata, Ludwigia octovalvis di Kebun Raya Purwodadi.

Masy Biodiv, 9(1), 53-58. https://doi.org/10.13057/psnmbi/m090108

[3] Chrisnawati, L., Mumtazah, D. F., Sari, D. M. (2022). Pelatihan

budidaya microgreens sebagai alternatif urban farming.

Communnity Development Journal, 3(2), 644-648.

https://doi.org/10.31004/cdj.v3i2.4418

[4] Darma, W & Marpaung, M. P. (2020). Analisis jenis dan kadar

saponin ekstrak akar kuning (Fibraurea chloroleuca Miers)

secara gravimetri. Jurnal Pendidikan Kimia dan Ilmu Kimia,

3(1), 51-59. https://doi.org/10.31602/dl.v3i1.3109

[5] Ezeonu, C. S., & Ejikeme, C. M. (2016). Qualitative and

quantitative determination of phytochemical contents of indigenous Nigerian softwoods. New Journal of Science,

2016(5601327) 1-9. https://doi.org/10.1155/2016/5601327

[6] Haq, S. H., Gusmalawati, D., & Ifadatin, S. (2024).

Perkecambahan biji sawi (Brassica juncea (L.) Czern.) varietas

tosakan yang kedaluarsa berdasarkan bahan priming dan lama

waktu perendaman. Jurnal Teknologi Terapan, 8(2), 797–806.

https://doi.org/10.33379/gtech.v8i2.4033

[7] Hasan, P. A., Sari, A. P & Alfiansya. (2023). Pengaruh ukuran

biji, suhu dan lama perendaman terhadap perkecambahan biji

kacang merah (Phaseolus vulgaris). Jurnal Matematika, Sains

[14] Leverett, L. D. (2017). Germination phenology determines the

propensity for facilitation and competition. Ecology, 98(9),

2437-2446. https://www.jstor.org/stabil/26601095

[15] Pompelli, M.F., Jarma-Orozco, A., Rodriguez-Páez, L.A.

(2023). Imbibition and germination of seeds with economic

and ecological interest: Physical and biochemical factors

involved.Sustainability, 15, 5394.https://doi.org/10.3390/su15065394

Pembelajarannya, 9(1), 95-100. https://doi.org/10.31605/saintifik.v9il.389

[16] Silla, W., Hendrik, A. C., & Nitsae, M. (2020). Identifikasi dan

penapisan alkaloid pada jenis-jenis tumbuhan paku

(Pteridophyta) di cagar alam gunung mutis. Jurnal Pendidikan

dan Sains Biologi, 3(3), 102-110.

[8] Hasanah, N. (2015). Aktivitas antioksidan ekstrak etanol daun

salam. Jurnal Pena Medika: Jurnal Kesehatan, 5(1), 55-99.

https://jurnal.unikal.ac.id/index.php/medika/article/viewFil

e/345/325

[9] Hersila, N., Chatri, M., Vauzia & Irdawati. (2023). Senyawa

metabolit sekunder (tanin) pada tanaman sebagai antifungi.

Jurnal Embrio, 15(1), 16-22. https://doi.org/1031317/embrio

[10] Hidayat, M. A., Fitri, A., & Kuswandi, B. (2017). Scanometry

as microplate reader for high throughput method based on

DPPH dry reagent for antioxidant assay. Acta Pharmaceutica

Sinica https://doi.org/10.33323/indigenous.v3i3.129

B, 7(3), 395–400.

[17] Stefani, S., & Andayani, E.D. (2022). Anti aging benefits of

microgreen. Journal of Medicine and Health, 4(2), 190–202.

https://doi.org/10.28932/jmh.v4i2.3887

[18] Walters, C. (2015). Orthodox and recalcitrant seed biology: A

continuum between extremes. Planta, 2 (42), 257–269.

https://doi.org/10.1007/s00425-015-2312-6

[19] Xiao, Z., Codling, E.E., Luo, Y., Nou, X., Lester, G.E., &

Wang, Q. (2016). Microgreens of Brassicaceae: Mineral

composition and content of 30 varieties. Journal of Food

Composition and Analysis. 49, 87–93.

https://doi.org/10.1016/j.apsb.2017.02.001

https://doi.org/10.1016/j.jfca.2016.04.006

[11] Ikrarwati, F., Zulkarnaen, I., Fathonah, A., Nurmayulis, F., &

Eris, F. R. (2020). Pengaruh jarak lampu LED dan jenis media

tanam terhadap microgreen basil (Ocimum basilicum L.).

Teaching Factory, 15–25. https://doi.org/10.25047/agropross.2020.7

[20] Xiao, Z., Lester, G. E., Luo, Y., & Wang, Q. (2012).

Assessment of vitamin and carotenoid concentrations of

emerging food product: Edible microgreens. Journal of

Agricultural and Food Chemistry, 60, 7644-7651.

https://doi.org/10.1021/jf300459b

[21] Yang, L., Wen, K. S., Ruan, X., Zhao, Y. X., Wei, F., & Wang,

Q. (2018). Response of plant secondary metabolites to

environmental

[12] Kalemba, M. R. K., Makhuvele, R., & Njobeh, P. B. (2024).

Phytochemical screening, antioxidant activity of selected

methanolic plant extracts and their detoxification capabilities

against AFB₁ toxicity. Heliyon, 10 (2), 24435.

https://doi.org/10.1016/j.heliyon.2024.e24435

[13] Kyriacou, M. C., El-Nakhel, C., Pannico, A., Graziani, G.,

Soteriou, G. A., Giordano, M., & Rouphael, Y. (2016).

Functional quality in novel food sources: Genotypic variation

in the nutritive and phytochemical composition of

microgreens. Food Chemistry, 199, 492–502.

https://doi.org/10.1016/j.foodchem.2015.12.011

factors. Molecules, 23(4), 762. https://doi.org/10.3390/molecules23040762

[22] Yora, M., Ilham, D. J., Rahayu, S. N. C., Putri, Z. T. (2024).

Acceleration of rooting ability and root development of coffee

seedlings (Coffea canephora L.) through a combination of

dormancy breaking techniques aand coffee types. Jurnal

Biologi Tropis, 24(1), 617-524. https://doi.org/10.29303/jbt.v24i1b.8197

Published

2026-04-30

How to Cite

Oktari, H., Warnita, W. and Budi Setiawan, R. (2026) “Germination Timing, Plant Chemicals Screening and Assessment of Antioxidant Activity on Various Microgreens Vegetables Employing the DPPH (2,2-diphenyl-1- picrylhydrazyl) Technique ”, JERAMI : Indonesian Journal of Crop Science, 8(3), pp. 119–124. doi: 10.25077/jijcs.v8i3.247.

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