Non‑Toxic Effects of Forest Soil‑Derived Microbial Inoculants on the Growth of Kepok Tanjung Banana Seedlings during the Ex Vitro Phase
DOI:
https://doi.org/10.25077/jijcs.v8i3.220Keywords:
Microbial Inoculant, Tanjung Kepok Banana, Bacterial and Fungal Consortium, Tissue Culture, Sustainable AgricultureAbstract
Kepok tanjung banana (Musa paradisiaca var. balbisiana) is a priority commodities that has challenges in conventional seedling development because it is susceptible to disease. This study aims to evaluate the effect of non-toxic microbial inoculants isolated from tropical rainforest soil on the growth of Kepok tanjung banana seedlings in tissue culture seedlings. The method used was an experiment with a completely randomized design (CRD) involving a control and various microbial inoculation treatments (bacteria, fungi, and microbial consortium). Results showed that treatments SP8 and SP3 (bacteria) gave the highest stem height, 88.5 cm and 87.0 cm, respectively, and the largest stem diameter (SP3: 27.2 mm). In addition, the highest leaf length was found in SP8 (49.0 cm) with greener leaf color (score 5). All microbial treatments showed non-toxic results, with media pH remaining stable (6.3-6.6). The bacterial and fungal consortium (KJB) treatment, showed better results than single inoculants in improving nutrient availability and pathogen control. This research supports sustainable agriculture and contributes to the SDGs, particularly SDG 2 (Zero Hunger) and SDG 12 (Responsible Consumption and Production) with an environmentally friendly solution for the efficient and sustainable propagation of banana seedlings.
Downloads
References
[1] Chen, Z., Zhang, M., Li, X., & Liu, H. (2022). Impact of microbial inoculants on soil health and plant growth in banana cultivation systems: A review. Journal of Soil Science and Plant Nutrition, 22 (7), 453-464. https://doi.org/10.1080/02243707.2022.2021787
[2] Compant, S., Samad, A., Faist, H., & Sessitsch, A. (2019). A review on the plant microbiome: Ecology, functions, and emerging trends in microbial application. Journal of Advanced Research, 19, 29–37. https://doi.org/10.1016/j.jare.2019.03.004
[3] Díaz-Rodríguez, A. M., Parra Cota, F. I., Cira Chávez, L. A., García Ortega, L. F., Estrada Alvarado, M. I., Santoyo, G., & de los Santos-Villalobos, S. (2025). Microbial Inoculants in Sustainable Agriculture: Advancements, Challenges, and Future Directions. Plants, 14 (2), 191. https://doi.org/10.3390/plants14020191
[4] dos Reis, G. A., Martínez-Burgos, W. J., Pozzan, R., Pastrana Puche, Y., Ocán-Torres, D., de Queiroz Fonseca Mota, P., Rodrigues, C., Lima Serra, J., Scapini, T., Karp, S. G., & Soccol, C. R. (2024). Comprehensive Review of Microbial Inoculants: Agricultural Applications, Technology Trends in Patents, and Regulatory Frameworks. Sustainability (Switzerland), 16 (19), 8720. https://doi.org/10.3390/su16198720
[5] Febmita, E., & Putri, S. D. (2023). Test of Several Natural Growth Regulators for Vegetative Propagation of Banana (Musa paradisiaca L.) Variety Kepok Tanjung. Agroplasma, 10(1), 216–226.
[6] Hajji-Hedfi, L., Dali, S., Rhouma, A., Al-Ani, L. K. T., Chihani-Hammas, N., Khlif, A., & Bargougui, O. (2025). Microbial inoculants for plant resilience performance: roles, prospects and challenges. Journal of Plant Diseases and Protection, 132 (2). https://doi.org/10.1007/s41348-025-01080-7
[7] Hasan, A., Tabassum, B., Hashim, M., & Khan, N. (2024). Role of Plant Growth Promoting Rhizobacteria (PGPR) as a Plant Growth Enhancer for Sustainable Agriculture: A Review. Bacteria, 3 (2), 59-75. https://doi.org/10.3390/bacteria3020005
[8] Jiyang, L., Gui, D., Zhang, U., Li, R., Chen, X., & Sha, Z. (2022). Field application of microbial inoculants improved crop foliar morphology and physiology performance: A global meta-analysis. Scientia Horticulturae, 326 , 112769. https://doi.org/https://doi.org/10.1016/j.scienta.2023.112769
[9] Kumar, A., Sahoo, S., & Mishra, B. (2024). Microbial inoculants in agricultural production: Enhancing plant growth and soil fertility. Agricultural Research Journal, 12 (1), 33-45. https://doi.org/10.1016/j.agres.2023.11.012
[10] Naz, M., Dai, Z., Hussain, S., Tariq, M., Danish, S., Khan, I. U., & Du, D. (2022). The soil pH and heavy metals revealed their impact on soil microbial community. Journal of Environmental Management, 321, 115770. https://doi.org/https://doi.org/10.1016/j.jenvman.2022.115770
[11] Putri, S. D., Hermansah, H., Agustian, A., & Nurmiati, N. (2024). Literature Review: The Role Of Tropical Rainforest Soil Microorganisms In Organic Matter Decomposition To Improve The Quality Of Planting Media For Seedlings.. 47(2), 645–659.
[12] Singh, A. P., & Khan, S. (2024). Microbial Inoculants: Effective Biofertilizers for Improving Crop Productivity. International Journal of Environmental Sciences & Natural Resources, 34 (3), 556390. https://doi.org/10.19080/ijesnr.2024.34.556390
[13] Singh, M., Gopal, J., & Bhoopander, S. (2023). Microbial inoculants alter resilience towards drought stress in wheat plants. Plant Growth Regulation, 101 (3), 823-843. https://doi.org/10.1007/s10725-023-01059-0
[14] Timofeeva, A. M., Galyamova, M. R., & Sedykh, S. E. (2023). Plant Growth-Promoting Bacteria of Soil: Designing of Consortia Beneficial for Crop Production. Microorganisms, 11 (12), 2864. https://doi.org/https://doi.org/10.3390/microorganisms11122864
[15] Xiang, D., Yang, X., & Liu, B. (2023). Biological management of Fusarium wilt in banana using microbial agents. Microbial Biotechnology, 14 (2), 172-182. https://doi.org/10.3389/fmicb.2023.809872.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Santi Diana Putri, Hermansah, Agustian, Nurmiati

This work is licensed under a Creative Commons Attribution 4.0 International License.
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.



