The Application Of PCR RAPD Technique In Determining Female Sex Of Kapulasan (Nepheleum Lapaceum Mutabile)
Abstract
Kapulasan is one of horticultural plants derived from tropical region that has significant economic value. One of the obstacles in cultivating the plant is that kapulasan has flowering type of androdioceus, i.e. the male and the female flowers are separated. Female flowers produce hermaphroditic flowers that can produce fruits while the male ones will not produce fruits. In the cultivation of the plant, it is very difficult to determine the female flowers if they are propagated by seeds. It takes up to 7 years to determine the nature of the flowering. One of the important steps that can be done is through a system of early detection using molecular marks. RAPD technique as a simple technique can be used to distinguish the characteristics. The results of the research show that of the 250 primers used, the OPM-09 primer produces female sex bands related to the controller of the female kapulasan plants of which the ribbon produced is 512 bp in size.
References
Durand R. and Durand B. 1990. Sexual determination and sexual differentiation. Critical Review of Plant Science 9:295-316.
George. J and A. Karun, 2011. Marker assisted detection of seed sex ratio in Palmyrah palm (Borassus flabellifer L.). Reseacrh Comunication Current Science: 100 (6). P. 922-925.
Hormaza, J.I. 1994. An analysis of sex expression, geographic distribution and genetic relatedness among clones and cultivars of pistachio (Pistacia vera L.). PhD Thesis, University of California, Davis.
Manoj, P., Banerjee, N.S., Ravichandran, P., 2005. Plant Genet. Res. Newsletter 141, 44-50.
Michelmore, R.W., I. Paran and R.V. Kesseli, 1991. Identification of markers linked to disease resistance genes by bulked segregation analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. USA, 88: 9829-9832.
Prastowo, N.H, Roshetko, J.M, Maurung, G.E.S. Nugraha, E. Tukan, J.M. Harum, F. 2006. Tehnik embibitan dan Perbanyakan Vegetatif Tanaman Buah. World Agroforestry Centre (ICRAF) & Winrock International. 100 p.
Sing. M, S. Kumar, A.K. Sing, D. Ram, and G. Kalloo. 2002. Female sex associated RAPD marker in point gourd (Trichosanthes dioica Roxb.) Reseacrh Comunication. Current Science: 82 (2). P. 131-132.
Uji. T. 2000. Keanekaragaman jenis buah-buahan hutan di Taman Nasional Bogani Nani Wartabone, Sulawesi Utara. Prosiding Seminar Hak Cipta Puspa Satwa Nasional, Kebun Raya Bogor 5 November 2000. Balitbang Biologi LIPI. 5 hal.
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