Micropropagationof Male and Female Trees of Andaleh (MorusmacrouraMiq.) through In-vitro Culture using Several Compositionsof Basal Medium

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Aswaldi Anwar

Abstract

Andalehis the local name of  MorusmacrouraMiq. in West Sumatra, Indonesia. Nowadays, this dioeciously speciesis in endangered situation. The aim of the research is to find out the appropriate combination of plant growth regulator to induce shoot multiplication of explants from male and female trees of andaleh. The plantlets from this research will be used in the next future to conserve this endangered species in vitro and in vivo, especially in preparing parental material in breeding program. Young buds from male and female trees were used as an explants in basal medium Murashige and Skoog supplemented with BAP (0.5, 1.0, 1.5 and 2.0 mg.L-1) in combination with NAA (1.0 mg.L-1 for each).  The frequency of bud break was 50 % in MS medium supplemented with BAP (0.5 mg.L-1) and NAA 1.0 mg.L-1 for both source of explants (female and female trees of andaleh) after 3 weeks of culture. Generally, the number of shoot induction was very low. On the other hand, the rate of callus formation was high (100%) in highest BAP concentration (2.0 mg.L-1).

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How to Cite
Anwar, A. (2018, August 30). Micropropagationof Male and Female Trees of Andaleh (MorusmacrouraMiq.) through In-vitro Culture using Several Compositionsof Basal Medium. JERAMI Indonesian Journal of Crop Sciences, 1(1), 32-38. Retrieved from http://jerami.faperta.unand.ac.id/index.php/Jerami-JIJCS/article/view/13
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References

Akram, M.andAftab, F., 2012.Efficient Micropropagationand Rooting of King White Mulberry (MorusmacrouraMiq.) var. Laevigata from Nodal Explants of Mature Tree.Pakistan Journal of Botany, 44, pp. 285-289.
Anis, M., Faisal, M.and Singh, S.K.,2003.Micropropagation of Mulberry (MorusalbaL.) throughIn-vitro Culture of Shoot Tip and Nodal Explants. Plant Tissue Culture, 13 (1), pp. 47-51.
Benedetta, C., Germana, P. and Germana, M. A., 2007. In-vitro Response of Two Sicilian Genotypes of Morus (L.) through Axillary Bud Culture. Caryologia, 60, pp. 178–181.
Chitra, D.V. and Padmaja, G., 2002. Seasonal Influence on Axillary Bud Sprouting and Micropropagation of Elite Cultivars of Mulberry. Scientia Horticulturae, 92(1), pp.55-68.
Chitra, D. S. V., Chinthapalli, B. andPadmaja, G., 2014. Efficient Regeneration System for Genetic Transformation of Mulberry (Morus indica L. cultivar ‘S-36’) using In-vitro Derived Shoot Meristems. American Journal of Plant Sciences, 5, pp. 1–6.
Feyissa, T., Welander, M. and Negash, L., 2005. Micropropagation of Hagenia abyssinica: AMultipurpose Tree. Plant Cell, Tissue and Organ Culture, 80(2), pp.119-127.
Kapur, A., Bhatnagar, S., Khurana, P. and Rao, E., 2001. Efficient Regeneration from Mature Leaf Explants of Indian Mulberry via Organogenesis. Sericologia, 41, pp. 1-8(France).
Sita, G. L., 1982. Tissue Culture of Eucalyptus species. Pp.180-184. In: Tissue Culture of Economically Important Plants: Proceedings of the International Symposium held at the Botany Department, National University of Singapore, Singapore. (Ed.) A. N. Rao.
Lalitha, N., Kih, S., Banerjee, R., Chattopadhya, S., Saha, A.K. and Bindroo, B.B., 2013. High Frequency Multiple Shoot Induction and In-vitro Regeneration of Mulberry (Morus indica L. cv. S-1635). International Journal of Advanced Research, 1(1), pp.22-26.
Miquel, F.A.W, 1862.Sumatra III Zizneplantenwereld. Amsterdam.
Mogea, P.J., Gandawidjaya,D. J.,Wiriadinata, H.,Nasution, R. and Irawati, 2001. Rare Plants of Indonesia. Puslitbang LIPI, Herbarium Bogoriense, Bogor.
Murashige, T. and Skoog, F., 1962. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15(3), pp.473-497.
Narayan, P., Chakraborty, S. and Rao, G.S., 1989. Regeneration of Plantlets from the Callus of Stem Segments of Mature Plants of Morus alba L. Proceedings of the Indian National Science Academy, 5, p.469.
Ohyama, K. and Oka, S., 1987. Mulberry. In Cell and Tissue Culture in Forestry (pp. 272-284). Springer, Dordrecht.
Pattnaik, S.K. and Chand, P.K., 1997. Rapid Clonal Propagation of Three Mulberries, Morus cathayana Hems. L., M. lhou Koiz. andM. serrata Roxb., through In-vitro Culture of Apical Shoot Buds and Nodal Explants from Mature Trees. Plant Cell Reports, 16(7), pp.503-508.
Syamsuardi, 2015.Morphological and GeneticDiversity of AndalasTree (MorusmacrouraMiq.),IdentityFlora of West Sumatra, and ItsSustainableUtilization.In: Seminar Proceeding of Workshop on StrengtheningAppreciation on Conservation of WoodyPlant of Sumatra. Pekanbaru, Indonesia.
Thomas, T.D., 2003. Advances in Mulberry Tissue Culture. Journal ofPlantBiology,45, pp. 7-21.
Vijayan, K., Chakraborti, S.P. and Roy, B.N., 2000. Plant Regeneration from Leaf Explants of Mulberry: Influence of Sugar, Genotype and 6-Benzyladenine. Indian Journal of Experimental Biology, 38, pp. 504-508.
Vijayan, K., Saratchandra, B. and da Silva, J.A.T., 2011. Germplasm Conservation in Mulberry (Morus spp.). Scientia Horticulturae, 128(4), pp.371-379.
Vijayan, K., Raju, P.J., Tikader, A. and Saratchnadra, B., 2014. Biotechnology of Mulberry (Morus L.)-A Review. Emirates Journal of Food and Agriculture, 26(6), p.472.
Zaki, M., Kaloo, Z.A. and Sofi, M.S., 2011. Micropropagation of Morus nigra L. from Nodal Segments with Axillary Buds. World Journal of Agricultural Sciences, 7(4), pp.496-503.